Instruments

BD FACSAria Cell Sorting System( Flow cytometer)

The BD FACSAria instrument is the first benchtop flowcytometer with sorter that incorporates a fixed-alignment cuvette flow cell. This new flow cell provides superior fluorescence sensitivity. The fixed optical system offers freedom from instrument maintenance and alignment. It accommodates two air-cooled lasers at 488-nm, 633-nm and analyse 9 parameters simultaneousely. It can achieve Digital acquisition rates of up to 70,000 events/second. At present we are performing following tests on this machine:

1. Leukemia immunophenotyping
2. Diagnosis of Paroxysmal Haemoglobinuria
3. Analysis of major and minor lymphocyte subsets
4. Diagnosis of platelet function defects
5. Diagnosis of different primary immunodeficiency disorders
6. Diagnosis of red cell membrane defects
7. Characterization of haematopoietic and mesenchymal stem cells
8. Sorting of fetal NRBCs from maternal blood
9. Sorting of lymphocytes and neutrophils
10. Sorting of CD34 positive cells